Antimicrobial resistance in fungi

The Allshire lab uses fission yeast, Schizosaccharomyces pompe, to study the process of heterochromatin silencing and how this is may influence the epigenetic factors implicated in the development of antifungal resistance.

Robin is interested in whether epigenetic heterochromatin mutants are linked to common pathways of drug resistance (e.g. it is thought that azole-based fungicides used on crops target same pathways as human anti-fungals).

Can we use our knowledge of epigenetic regulation to intervene and reduce resistance to anti-fungals in the future?

Allshire lab website

The Hardwick lab is interested in the process of Cryptococcus infection and the role of polyploidy in a stage of fungal cell development (so-called Titan cells) in the lung.

They are using flow cytometry to monitor ploidy transitions (i.e. making daughter cells) and the influence of cell cycle checkpoint inhibitors such as spindle checkpoint kinases.

They are interested in whether these kinase inhibitors, in combination with anti-microtubule inhibitors, might make an effective combination treatment for this deadly infection.

Hardwick lab website

Genetic plasticity in the human fungal pathogen, C. albicans is instrumental to drive adaptation to the host environment.

Chromosome instability driven by Candida-specific cohesin’s regulation could be one of the means to achieve this genetic plasticity.

Vasso is interested in addressing how cohesin is loaded, maintained and dissolved in this fungus when challenged under different environmental stresses.  SHe is starting her own lab, with the ultimate goal is that novel insights into the mechanisms controlling the function of cohesins in C. albicans could guide the development of specific antifungal drugs.

The Wallce lab is interested in the role of messenger RNA in how cells adapt to their environment.

They are studying how a fungal virulence factor (Ssd1) controls translation of mRNAs affecting cell wall growth in fungi, and are interested in how similar translation regulatory factors help to build the cell wall and contribute to antifungal responses. 

Edward is an expert in ribosome profiling for the high-throughput study of translation, for example identifying a large number of alternative translation events in Cryptococcus that may generate alternative protein isoforms. To study these processes in mechanistic detail, the Wallace lab are building a synthetic biology / reporter gene toolkit for Cryptococcus.

Wallace lab website

Lorraine Pfavayi, a PhD student in Francisca Mutapi's lab is interested in the incidence and prevalence of fungal infections in Zimbabwe.

There is a wide spectrum of fungal diseases from those causing superficial skin infections to more chronic fungal diseases. Lorraine is gathering data from a wide range of sources to quantify the burden as well as raise awareness of these fungal infections which are often under reported.

Mutapi lab website